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A chiral auxiliary is a stereogenic group or unit that is temporarily incorporated into an organic compound in order to control the stereochemical outcome of the synthesis. The chirality present in the auxiliary can bias the stereoselectivity of one or more subsequent reactions. The auxiliary can then be typically recovered for future use.
General scheme for employing a chiral auxiliary in asymmetric synthesis
Most biological molecules and pharmaceutical targets exist as one of two possible enantiomers; consequently, chemical syntheses of natural products and pharmaceutical agents are frequently designed to obtain the target in enantiomerically pure form. Chiral auxiliaries are one of many strategies available to synthetic chemists to selectively produce the desired stereoisomer of a given compound.
Chiral auxiliaries were introduced by E.J. Corey in 1975 with chiral 8-phenylmenthol and by B.M. Trost in 1980 with chiral mandelic acid. The menthol compound is difficult to prepare and as an alternative trans-2-phenyl-1-cyclohexanol was introduced by J. K. Whitesell in 1985.
Chiral auxiliaries are incorporated into synthetic routes to control the absolute configuration of stereogenic centers. David Evans' synthesis of the macrolide cytovaricin, considered a classic, utilizes oxazolidinone chiral auixiliaries for one asymmetric alkylation reaction and four asymmetric aldol reactions, setting the absolute stereochemistry of nine stereocenters.
Cytovaricin, synthesized by D. A. Evans in 1990. Blue and red bonds indicate stereocenters set with the use of chiral auxiliaries.
A typical auxiliary-guided stereoselective transformation involves three steps: first, the auxiliary is covalently coupled to the substrate; second, the resulting compound undergoes one or more diastereoselective transformations; and finally, the auxiliary is removed under conditions that do not cause racemization of the desired products. The cost of employing stoichiometric auxiliary and the need to spend synthetic steps appending and removing the auxiliary make this approach appear inefficient. However, for many transformations, the only available stereoselective methodology relies on chiral auxiliaries. In addition, transformations with chiral auxiliaries tend to be versatile and very well-studied, allowing the most time-efficient access to enantiomerically pure products.
Furthermore, the products of auxiliary-directed reactions are diastereomers, which enables their facile separation by methods such as column chromatography or crystallization.
In an early example of the use of a chiral auxiliary in asymmetric synthesis, E.J. Corey and coworkers conducted an asymmetric Diels-Alder reaction between (-)-8-phenylmenthol acrylateester and 5-benzyloxymethylcyclopentadiene. The cycloaddition product was carried forward to the iodolactone shown below, an intermediate in the classic Corey synthesis of the prostaglandins. It is proposed that the back face of the acrylate is blocked by the auxiliary, so that cycloaddition occurs at the front face of the olefin.
Diastereoselective Diels-Alder cycloaddition with the chiral auxiliary (-)-8-phenylmenthol in route to the prostaglandins.
(-)-8-phenylmenthol can be prepared from either enantiomer of pulegone,
though neither route is very efficient. Because of the widespread utility of the 8-phenylmenthol auxliliary, alternative compounds that are more easily synthesized, such as trans-2-phenyl-1-cyclohexanol
and trans-2-(1-pheyl-1-methylethyl)cyclohexanol have been explored.
Hisashi Yamamoto first utilized (R)-BINOL as a chiral auxiliary in the asymmetric synthesis of limonene, which is an example of cyclic mono-terpenes. (R)-BINOL monoeryl ether was prepared by the monosilylation and alkylation of (R)-BINOL as the chiral auxiliary. Followed with the reduction by organoaluminum reagent, limonene was synthesized with low yields (29% yield) and moderate enantiomeric excesses up to 64% ee.
First utilization of BINOL as a chiral auxiliary.
The preparation of a variety of enantiomerically pure uncommon R-amino acids can be achieved by the alkylation of chiral glycine derivatives possessing axially chiral BINOL as an auxiliary. It has been depicted by Fuji et al. Based on different electrophile, the diastereomeric excess varied from 69% to 86.
Diastereoselective addition between Grignard and BINOL protected aldehyde
Protected at the aldehyde function with (R)-BINOL, arylglyoxals reacted diastereoselectively with Grignard reagents to afford protected atrolactaldehyde with moderate to excellent diastereomeric excess and high yields.
Diastereoselective addition between Grignard and BINOL protected aldehyde
trans-2-Phenylcyclohexanol was used in ene reaction as a chiral auxiliary.
In the total synthesis of (-)-Heptemerone B and (-)-Guanacastepene E, Attached with trans-2-pheynlcyclohexanol, the glyoxylate reacted with 2,4-dimethyl-2-pentene, in the presence of tin(IV) chloride, yielding the desired anti adduct as the major product, together with a small amount of its syn isomer with 10:1 diastereomeric ratio.
The glyoxylate reacted with 2,4-dimethyl-2-pentane with trans-2-phenylcyclohexanol as a chiral auxiliary
Chiral oxazolidinones have been employed most widely in stereoselective aldol reactions.
Soft enolization with the Lewis acid dibutylboron triflate and the base diisopropylethylamine gives the (Z)-enolate, which undergoes a diastereoselective aldol reaction with an aldehyde substrate. The transformation is particularly powerful because it establishes two contiguous stereocenters simultaneously.
Stereoselective Evans aldol reaction.
A model for the observed stereoselectivity can be found below. The syn-stereorelationship between the methyl group and the new secondary alcohol results from a six-membered ring Zimmerman-Traxler transition state, wherein the enolate oxygen and the aldheyde oxygen both coordinate boron. The aldehyde is oriented such that the hydrogen is placed in a pseudo-axial orientation to minimize 1,3-diaxial interactions. The absolute stereochemistry of the two stereocenters is controlled by the chirality in the auxiliary. In the transition structure, the auxiliary carbonyl is oriented away from the enolate oxygen so as to minimize the net dipole of the molecule; one face of the enolate is blocked by the substituent on the chiral auxiliary.
Model for the stereoselectivity of an Evans aldol reaction.
A variety of transformations have been developed to facilitate removal of the oxazolidinone auxiliary to generate different synthetically useful functional groups.
Transformation of an oxazolidinone imide to different functional groups.
Camphorsultam, or Oppolzer's sultam, is a classic chiral auxiliary.
In the total synthesis of manzacidin B, Ohfune group utilized camphorsultam to construct the core oxazoline ring asymmetrically. Comparing with oxazolidinone as the chiral auxiliary, camphorsultam had a significant (2S,3R)-selectivity.
The chiral camphorsultam was found to be a superior chiral auxiliary to the oxazolidinone in view of the single asymmetric induction.
Camphorsultam also acts as a chiral auxiliary in Michael addition. Lithium base promoted stereoselective Michael addition of thiols to N-mcthacryloylcamphorsultam produced the corresponding addition products in high diastereoselectivity.
Camphorsultam was used as a chiral auxiliary in Michael addition.
Camphorsultam was used as a chiral auxiliary for the asymmetric Claisen rearrangement. In the presence of butylated hydroxytoluene (BHT) used as a radical scavenger, a toluene solution of the adduct between geraniol and camphorsultam was heated in a sealed tube at 140 °C, to provide mainly the (2R,3S)-isomer as the major rearrangement product in 72% yield, securing the two contiguous stereocenters including the quaternary carbon.
Camphorsultam was used as a chiral auxiliary in Claisen rearrangement.
The ?-proton of the carbonyl compound is easily deprotonated by a non-nucleophilic base to give the enolate, which can further react. The configuration of the addition compound, such as with an alkyl halide, is directed by the methyl group. Thus, any addition product will be syn with the methyl and anti to the hydroxyl group. The pseudoephedrine chiral auxiliary is subsequently removed by cleaving the amide bond with an appropriate nucleophile.
Both enantiomers of pseudoephedrine are commercially available. Racemic pseudoephedrine is marketed as Sudafed - a nasal decongestant. Because pseudoephedrine can be used to illegally make methamphetamine, the purchase of pseudoephedrine for use in academic or industrial research is rather regulated. As an alternative, Myers et. al reported the utility of pseudoephenamine chiral auxiliaries in alkylation reactions. While pseudoephenamine is not readily available from commercial sources, it can be synthesized with relative ease from benzil and cannot be used to make amphetamines.
Pseudoephedrine and pseudoephenamine chiral auxiliaries.
Acylation of (S,S)-psueodphedrine with an acid anhydride.
Pseudoephedrine amides undergo deprotonation by a strong base such as lithium diisopropylamide (LDA) to give the corresponding (Z)-enolates. Alkylation of these lithium enolates proceeds with high facial selectivity.
Diastereoselective alkylation of a pseudoephedrine amide.
The diastereoselectivity is believed to result from a configuration wherein one face of the lithium enolate is blocked by the secondary lithium alkoxide and the solvent molecules associated with that lithium cation. In accordance with this proposal, it has been observed that the diastereoselctivity of the alkylation step is highly dependent on the amount of lithium chloride present and on the solvent, tetrahydrofuran (THF). Typically, 4 to 6 equivalents of lithium chloride are sufficient to saturate a solution of enolate in THF at the reaction molarity.
Model for the diastereoslective alkylation of a pseudoephedrine amide enolate.
One primary advantage of asymmetric alkylation with pseudoephedrine amides is that the amide enolates are typically nucleophilic enough to react with primary and even secondary halides at temperatures ranging from -78 °C to 0 °C. Construction of quaternary carbon centers by alkylation of ?-branched amide enolates is also possible, though the addition of DMPU is necessary for less reactive electrophiles.
Transformation of pseudoephedrine amides into synthetically useful functional groups.
After cleavage, the auxiliary can be recovered and reused.
This specific sulfinamide chiral auxiliary was initially developed by Jonathan A. Ellman, and its use has been explored extensively by his group. Thus, it is often referred to as Ellman's auxiliary or Ellman's sulfinamide.
The two possible enantiomers of tert-butanesulfinamide
Either enantiomer of tert-butanesulfinamide can be reached from tert-butyl disulfide in two steps: a catalytic asymmetric oxidation reaction gives the disulfide monooxidation product in high yield and enantiomeric excess. Treatment of this compound with lithium amide in ammonia affords optically pure inverted product.
Synthesis of tert-butanesulfinamide from readily available chemicals
Condensation of tert-butanesulfinamide with an aldehyde or ketone proceeds in high yield and affords only the (E)-isomer of the corresponding N-Sulfinyl imines.
Condensation of tert-butanesulfinamide with aldehydes and ketones
Synthesis of chiral amines
Addition of a Grignard reagent to a tert-butanesulfinyl aldimine or ketimine results in asymmetric addition to give the branched sulfinamide. The observed stereoselectivity can be rationalized by a six-membered ring transition structure, wherein both oxygen and nitrogen of the sulfinyl imine coordinate magnesium.
Addition of a Grignard reagent to a tert-butanesulfinyl aldimine
SAMP can be prepared in six steps from (S)-proline, and RAMP can be prepared in six steps from (R)-glutamic acid.
Preparation of SAMP and RAMP from commercially available materials.
Condensation of SAMP or RAMP with an aldehyde or ketone affords the (E)-hydrazine. Deprotonation with lithium diisopropylamide and addition of an alkyl halide affords the alkylated product. The auxiliary can be removed by ozonolysis or hydrolysis.
Condensation, alkylation, and cleavage with SAMP/RAMP chiral auxiliaries
Chiral auxiliaries in industry
Chiral auxiliaries are generally reliable and versatile, enabling the synthesis of a large number of enantiomerically pure compounds in a time-efficient manner. Consequently, chiral auxiliaries are often the method of choice in the early phases of drug development.
The HIV protease inhibitor Tipranavir is marketed for the treatment of AIDS. The first enantioselective medicinal chemistry route to Tipranavir included the conjugate addition of an organocuprate reagent to a chiral Michael acceptor. The chiral oxazolidinone in the Michael acceptor controlled the stereochemistry of one of two stereocenters in the molecule. The final, commercial route to Tipranavir does not feature a chiral auxiliary; instead, this stereocenter is set by an asymmetric hydrogenation reaction.
Synthetic strategies for setting a key stereocenter in Tipranavir.
The calcium salt of atorvastatin is marketed under the trade name Lipitor for the lowering of blood cholesterol. The first enantioselective medicinal chemistry route to atorvastatin relied on a diastereoselective aldol reaction with a chiral ester to set one of the two alcohol stereocenters. In the commercial route to atorvastatin, this stereocenter is carried forward from readily available isoascorbic acid.
Synthetic strategies for setting a key stereocenter in atorvastatin.
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