Its length is 256 km (159 mi). It is the third longest border of Guatemala after the borders that separate that country from Mexico and Belize. For Honduras, it is the shortest border, coming after those with Nicaragua and El Salvador.
The border begins at the mouth of the Motagua River in the Gulf of Honduras, then proceeds upstream. It continues towards the southwest, following several straight lines between geographic landmarks, divides between watersheds, and rivers and streams to its end at the tripoint with El Salvador at the summit of the Montechristo massif.
The border was fixed in 1933 by an arbitration tribunal in the United States after Nicaragua and Guatemala signed an arbitration treaty in 1930. A demarcation commission headed by Sidney H. Birdseye conducted an areal survey and erected 1,028 boundary markers between 1933 and 1936.