Hudson Street has two distinct one-way traffic patterns that meet at Abingdon Square, at the street's intersection with Eighth Avenue and Bleecker Street. The southern portion of Hudson Street carries northbound traffic and begins at the intersection of West Broadway and Chambers Street. At Abingdon Square, the traffic is directed onto Eighth Avenue. Meanwhile, the section of Hudson Street north of Abingdon Square runs from 14th Street to Eighth Avenue. At 14th Street, southbound traffic from Ninth Avenue splits off into this street. Just below 14th Street, it is one of the major streets in the Meatpacking District. At Abingdon Square, traffic on Hudson Street goes into Bleecker Street.
The former New York Mercantile Exchange building is located at the corner of Hudson and Harrison Street in TriBeCa. Other notable buildings on this stretch of Hudson Street include The Church of St. Luke in the Fields and its garden, the White Horse Tavern (notorious for being the bar where poet Dylan Thomas drank and collapsed before he died of alcohol poisoning), and the headquarters of radio station WQHT ("Hot 97"), which has been the site of several shootings including a gunfight between the entourages of 50 Cent and The Game in 2005. The street is also home to the U.S. headquarters of the Pearson-owned Penguin Group. The Money.net firm is located at 333 Hudson Street, maker of an alternative platform to the Bloomberg Terminal.
A bike lane is located in the roadway, connecting a bike lane in Ninth Avenue to one in Bleecker Street. In May 2019, it was announced that Hudson Street between Canal and Houston Streets would be reconstructed with expanded sidewalks and a new bike lane for $27 million.