Get Phylogenetic Comparative Methods essential facts below. View Videos or join the Phylogenetic Comparative Methods discussion. Add Phylogenetic Comparative Methods to your PopFlock.com topic list for future reference or share this resource on social media.
Phylogenetic Comparative Methods
Use of information on the historical relationships of lineages to test evolutionary hypotheses
Phylogenetic comparative methods (PCMs) use information on the historical relationships of lineages (phylogenies) to test evolutionary hypotheses. The comparative method has a long history in evolutionary biology; indeed, Charles Darwin used differences and similarities between species as a major source of evidence in The Origin of Species. However, the fact that closely related lineages share many traits and trait combinations as a result of the process of descent with modification means that lineages are not independent. This realization inspired the development of explicitly phylogenetic comparative methods. Initially, these methods were primarily developed to control for phylogenetic history when testing for adaptation; however, in recent years the use of the term has broadened to include any use of phylogenies in statistical tests. Although most studies that employ PCMs focus on extant organisms, many methods can also be applied to extinct taxa and can incorporate information from the fossil record.
PCMs can generally be divided into two types of approaches: those that infer the evolutionary history of some character (phenotypic or genetic) across a phylogeny and those that infer the process of evolutionary branching itself (diversification rates), though there are some approaches that do both simultaneously. Typically the tree that is used in conjunction with PCMs has been estimated independently (see computational phylogenetics) such that both the relationships between lineages and the length of branches separating them is assumed to be known.
Phylogenetic comparative approaches can complement other ways of studying adaptation, such as studying natural populations, experimental studies, and mathematical models. Interspecific comparisons allow researchers to assess the generality of evolutionary phenomena by considering independent evolutionary events. Such an approach is particularly useful when there is little or no variation within species. And because they can be used to explicitly model evolutionary processes occurring over very long time periods, they can provide insight into macroevolutionary questions, once the exclusive domain of paleontology.
Home range areas of 49 species of mammals in relation to their body size. Larger-bodied species tend to have larger home ranges, but at any given body size members of the order Carnivora (carnivores and omnivores) tend to have larger home ranges than ungulates (all of which are herbivores). Whether this difference is considered statistically significant depends on what type of analysis is applied
Testes mass of various species of Primates in relation to their body size and mating system. Larger-bodied species tend to have larger testes, but at any given body size species in which females tend to mate with multiple males have males with larger testes.
Phylogenetic comparative methods are commonly applied to such questions as:
Felsenstein proposed the first general statistical method in 1985 for incorporating phylogenetic information, i.e., the first that could use any arbitrary topology (branching order) and a specified set of branch lengths. The method is now recognized as an algorithm that implements a special case of what are termed phylogenetic generalized least-squares models. The logic of the method is to use phylogenetic information (and an assumed Brownian motion like model of trait evolution) to transform the original tip data (mean values for a set of species) into values that are statistically independent and identically distributed.
The algorithm involves computing values at internal nodes as an intermediate step, but they are generally not used for inferences by themselves. An exception occurs for the basal (root) node, which can be interpreted as an estimate of the ancestral value for the entire tree (assuming that no directional evolutionary trends [e.g., Cope's rule] have occurred) or as a phylogenetically weighted estimate of the mean for the entire set of tip species (terminal taxa). The value at the root is equivalent to that obtained from the "squared-change parsimony" algorithm and is also the maximum likelihood estimate under Brownian motion. The independent contrasts algebra can also be used to compute a standard error or confidence interval.
Phylogenetic generalized least squares (PGLS)
Probably the most commonly used PCM is phylogenetic generalized least squares (PGLS). This approach is used to test whether there is a relationship between two (or more) variables while accounting for the fact that lineage are not independent. The method is a special case of generalized least squares (GLS) and as such the PGLS estimator is also unbiased, consistent, efficient, and asymptotically normal. In many statistical situations where GLS (or, ordinary least squares [OLS]) is used residual errors ? are assumed to be independent and identically distributed random variables that are assumed to be normal
whereas in PGLS the errors are assumed to be distributed as
where V is a matrix of expected variance and covariance of the residuals given an evolutionary model and a phylogenetic tree. Therefore, it is the structure of residuals and not the variables themselves that show phylogenetic signal. This has long been a source of confusion in the scientific literature. A number of models have been proposed for the structure of V such as Brownian motionOrnstein-Uhlenbeck, and Pagel's ? model. (When a Brownian motion model is used, PGLS is identical to the independent contrasts estimator.). In PGLS, the parameters of the evolutionary model are typically co-estimated with the regression parameters.
PGLS can only be applied to questions where the dependent variable is continuously distributed; however, the phylogenetic tree can also be incorporated into the residual distribution of generalized linear models, making it possible to generalize the approach to a broader set of distributions for the response.
Phylogenetically informed Monte Carlo computer simulations
Data for a continuous-valued trait can be simulated in such a way that taxa at the tips of a hypothetical phylogenetic tree will exhibit phylogenetic signal, i.e., closely related species will tend to resemble each other.
Martins and Garland proposed in 1991 that one way to account for phylogenetic relations when conducting statistical analyses was to use computer simulations to create many data sets that are consistent with the null hypothesis under test (e.g., no correlation between two traits, no difference between two ecologically defined groups of species) but that mimic evolution along the relevant phylogenetic tree. If such data sets (typically 1,000 or more) are analyzed with the same statistical procedure that is used to analyze a real data set, then results for the simulated data sets can be used to create phylogenetically correct (or "PC") null distributions of the test statistic (e.g., a correlation coefficient, t, F). Such simulation approaches can also be combined with such methods as phylogenetically independent contrasts or PGLS (see above).
^ abFelsenstein, Joseph (January 1985). "Phylogenies and the Comparative Method". The American Naturalist. 125 (1): 1-15. doi:10.1086/284325. S2CID9731499.
^Harvey, Paul H.; Pagel, Mark D. (1991). The Comparative Method in Evolutionary Biology. Oxford: Oxford University Press. p. 248. ISBN9780198546405.
^O'Meara, Brian C. (December 2012). "Evolutionary Inferences from Phylogenies: A Review of Methods". Annual Review of Ecology, Evolution, and Systematics. 43 (1): 267-285. doi:10.1146/annurev-ecolsys-110411-160331.
^Weber, Marjorie G.; Agrawal, Anurag A. (July 2012). "Phylogeny, ecology, and the coupling of comparative and experimental approaches". Trends in Ecology & Evolution. 27 (7): 394-403. doi:10.1016/j.tree.2012.04.010. PMID22658878.
^ abGarland, T.; Dickerman, A. W.; Janis, C. M.; Jones, J. A. (1 September 1993). "Phylogenetic Analysis of Covariance by Computer Simulation". Systematic Biology. 42 (3): 265-292. doi:10.1093/sysbio/42.3.265.
^Freckleton, R. P.; Harvey, P. H.; Pagel, M. (December 2002). "Phylogenetic Analysis and Comparative Data: A Test and Review of Evidence". The American Naturalist. 160 (6): 712-726. doi:10.1086/343873. PMID18707460. S2CID19796539.
^Martins, Emilia P.; Garland, Theodore (May 1991). "Phylogenetic Analyses of the Correlated Evolution of Continuous Characters: A Simulation Study". Evolution. 45 (3): 534-557. doi:10.2307/2409910. JSTOR2409910. PMID28568838.
Ackerly, D. D. 1999. Comparative plant ecology and the role of phylogenetic information. Pages 391-413 in M. C. Press, J. D. Scholes, and M. G. Braker, eds. Physiological plant ecology. The 39th symposium of the British Ecological Society held at the University of York 7-9 September 1998. Blackwell Science, Oxford, U.K.
Brooks, D. R., and D. A. McLennan. 1991. Phylogeny, ecology, and behavior: a research program in comparative biology. Univ. Chicago Press, Chicago. 434 pp.
Cheverud, J. M.; Dow, M. M.; Leutenegger, W. (1985). "The quantitative assessment of phylogenetic constraints in comparative analyses: sexual dimorphism in body weight among primates". Evolution. 39 (6): 1335-1351. doi:10.2307/2408790. JSTOR2408790. PMID28564267.
Eggleton, P., and R. I. Vane-Wright, eds. 1994. Phylogenetics and ecology. Linnean Society Symposium Series Number 17. Academic Press, London.
Felsenstein, J. 2004. Inferring phylogenies. Sinauer Associates, Sunderland, Mass. xx + 664 pp.
Freckleton, R. P.; Harvey, P. H.; Pagel, M. (2002). "Phylogenetic analysis and comparative data: a test and review of evidence". American Naturalist. 160 (6): 712-726. doi:10.1086/343873. PMID18707460. S2CID19796539.
Maddison, D. R. (1994). "Phylogenetic methods for inferring the evolutionary history and process of change in discretely valued characters". Annual Review of Entomology. 39: 267-292. doi:10.1146/annurev.ento.39.1.267.
Maddison, W. P. (1990). "A method for testing the correlated evolution of two binary characters: Are gains or losses concentrated on certain branches of a phylogenetic tree?". Evolution. 44 (3): 539-557. doi:10.2307/2409434. JSTOR2409434. PMID28567979.
Maddison, W. P., and D. R. Maddison. 1992. MacClade. Analysis of phylogeny and character evolution. Version 3. Sinauer Associates, Sunderland, Mass. 398 pp.
Martins, E. P., ed. 1996. Phylogenies and the comparative method in animal behavior. Oxford University Press, Oxford. 415 pp.
Page, R. D. M., ed. 2003. Tangled trees: phylogeny, cospeciation, and coevolution. University of Chicago Press, Chicago.
Pagel, M. D. (1993). "Seeking the evolutionary regression coefficient: an analysis of what comparative methods measure". Journal of Theoretical Biology. 164 (2): 191-205. doi:10.1006/jtbi.1993.1148. PMID8246516.
Rezende, E. L., and T. Garland, Jr. 2003. Comparaciones interespecíficas y métodos estadísticos filogenéticos. Pages 79-98 in F. Bozinovic, ed. Fisiología Ecológica & Evolutiva. Teoría y casos de estudios en animales. Ediciones Universidad Católica de Chile, Santiago. PDF
Smith, R. J.; Cheverud, J. M. (2002). "Scaling of sexual size dimorphism in body mass: a phylogenetic analysis of Rensch's rule in primates". International Journal of Primatology. 23 (5): 1095-1135. doi:10.1023/A:1019654100876. S2CID42439809.