|The intersection of Spencer and Collins Street, looking North West toward Southern Cross Station|
|Length||2 km (1.2 mi)|
Running roughly north-south, Spencer Street forms the western edge of the original Hoddle Grid. To the north Spencer Street becomes Dynon Road, whilst to the south it becomes Clarendon Street after crossing the Spencer Street Bridge over the Yarra River.
Spencer Street was the site of the first permanent buildings in the settlement now called Melbourne. The home of John Batman was built on nearby Batman's Hill where he lived until his death and the early camps of Captain William Lonsdale and Charles La Trobe were located along the street.
Many buildings and structures on Spencer Street are listed on the Victorian Heritage Register and/or classified by the National Trust of Australia. Others are more modern and significant for their current or future function. These include:
Victoria's largest police station, the City West Police Centre, is located on Spencer Street. The Victoria Police Centre is set to open adjacent to it on Spencer Street as the new headquarters for Victoria Police in 2020.
Spencer Street is served primarily by the Southern Cross railway station and is both a major terminus for V/Line regional passenger trains and a major stop for metropolitan trains. The street is served by tram routes 11, 12, 35, 96 and 109 at its southern end and 30, 75 and 86 towards the north.
There have been reports of supposed sightings of the ghost of John Spencer, 3rd Earl Spencer roaming around the corner of Francis Street and Spencer Street. This local attraction has brought ghost hunters from around Australia to investigate the phenomena, and is a common stop for local ghost tours.